TRANSCRIPTION-COUPLED STRUCTURAL DYNAMICS OF TOPOLOGICALLY ASSOCIATING DOMAINS REGULATE REPLICATION ORIGIN EFFICIENCY

Transcription-coupled structural dynamics of topologically associating domains regulate replication origin efficiency

Abstract Background Metazoan cells only utilize a small subset of the potential DNA replication origins to duplicate the whole genome in each cell cycle.Origin choice is linked to cell growth, differentiation, and replication stress.Although various genetic and epigenetic signatures have been linked to the replication efficiency of origins, CELLULI

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Validation of a high-throughput fermentation system based on online monitoring of biomass and fluorescence in continuously shaken microtiter plates

Abstract Background An advanced version of a recently reported high-throughput fermentation system with online measurement, called BioLector, and its validation is presented.The technology combines high-throughput screening and high-information content by applying online Upright Vacuum Cleaner monitoring of scattered light and fluorescence intensit

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Exploring the host response in infected lung organoids using NanoString technology: A statistical analysis of gene expression data.

In this study, we used a three-dimensional airway "organ tissue equivalent" (OTE) model at an air-liquid interface (ALI) to mimic human airways.We investigated the effects of three viruses (Influenza A virus (IAV), Human metapneumovirus (MPV), and Parainfluenza virus type 3 (PIV3) on this model, incorporating various control conditions for data int

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